BlueGene Biotech Canine BCL2 Associated X Protein ELISA kit
ShanghaiBlueGene Biotech
Canine BCL2 Associated X Protein ELISA kit
E08B0032 Canine BCL2 Associated X Protein ELISA kit
The Canine BCL2 Associated X Protein ELISA kit is suitable for the detection of samples from Canine species. BCL2 Associated X Protein can also be called BAX, BCL2L4, Bax Zeta, Apoptosis regulator.
Specifications of Canine BCL2 Associated X Protein ELISA kit
Product Information |
|
E08B0032 |
|
Product Name |
Canine BCL2 Associated X Protein ELISA kit |
Species |
Canine |
Product Size |
48 Tests / 96 Tests |
Concentration |
5-100 ng/mL |
Sensitivity |
|
Principal |
Competitive ELISA |
Sample Volume |
100 ul |
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time |
90 minutes |
Platform |
Microplate Reader |
Conjugate |
HRP |
Detection Method |
Colorimetric |
Storage |
2-8°C |
Kit Components |
||
MATERIALS |
SPECIFICATION |
QUANTITY |
MICROTITER PLATE |
96 wells |
stripwell |
ENZYME CONJUGATE |
1 vial |
|
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
STANDARD B (0.5mL) |
5 ng/mL |
1 vial |
STANDARD C (0.5mL) |
10 ng/mL |
1 vial |
STANDARD D (0.5mL) |
25 ng/mL |
1 vial |
STANDARD E (0.5mL) |
50 ng/mL |
1 vial |
STANDARD F (0.5mL) |
100 ng/mL |
1 vial |
SUBSTRATE A |
6 mL |
1 vial |
SUBSTRATE B |
6 mL |
1 vial |
STOP SOLUTION |
6 mL |
1 vial |
WASH SOLUTION (100 x) |
10 mL |
1 vial |
BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
BAX ELISA kitapplies the competitive enzyme immunoassay technique utilizing an anti-BAX antibody and an BAX-HRP conjugate. The assay sample and buffer are incubated together with BAX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAX concentration since BAX from samples and BAX-HRP conjugate compete for the anti-BAX antibody binding site. Since the number of sites is limited, as more sites are occupied by BAX from the sample, fewer sites are left to bind BAX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentration in each sample is interpolated from this standard curve.
Quality Control on Canine BCL2 Associated X Protein ELISA BAXkit
Coefficient of Variance |
Intra Variation% <10% |
|
Inter Variation% <12% |
||
Recovery |
90-104% |
|
Linearity |
Diluent Ratio |
Range % |
1:02 |
89-105 |
|
1:04 |
87-106 |
|
1:08 |
84-109 |
|
Specificity/Cross-reactivity |
No significant cross-reactivity or interference between BAX and analogues was observed. |
Citations of Canine BCL2 Associated X Protein ELISA kit
E08B0032 has been referenced in the below publications:
Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.
We can also offer sorts of cho host cell protein elisa kitfor sale, if you have needs, please contact us.
Send product request
Other supplier products
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit | E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit is suitable for the detection of samples from Bov... | |
Expression Plasmids | cre expression plasmidare a kind of double-stranded, closed-loop DNA with a size range from 1kb to more than 200kb. All kinds of antibody expressio... | |
BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit | E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samp... | |
Rat Alpha Amylase ELISA kit | E02A0010 Rat Alpha Amylase ELISA kit Rat Alpha Amylase ELISAkit is suitable for the detection of samples from Rat species. Alpha Amylase can als... | |
Rat Active Caspase 3 ELISA kit | E02A0015 Rat Active Caspase 3 ELISA kit Rat caspase 3 elisa kitis suitable for the detection of samples from Rat species. Active caspase 3 can a... |
Same products
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | Seller: Bluegene Biotech | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA... | |
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | Seller: Bluegene Biotech | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1... | |
BlueGene Biotech Chicken Total Adiponectin ELISA kit | Seller: Bluegene Biotech | E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch... | |
BlueGene Biotech Bovine Decorin ELISA kit | Seller: Bluegene Biotech | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam... | |
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit | Seller: Bluegene Biotech | E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit... |